Targeting the total set of transcripts in a population of cells under given conditions, transcriptome sequencing reveals molecular mechanisms underlying specific biological processes based on the integrated study of both gene structure and function.
For species with genome reference, transcriptome resequencing is applicable to offer information of transcripts structure, alternative splice, and gene fusion. It also detects new transcripts by mapping reads to genome reference.
Transcriptome de novo sequencing is the method of sequencing the transcriptome of organisms or species based on next-generation sequencing technology and delivering unbiased information of transcriptome without any information of the interesting species.
Small RNA Sequencing
Small RNA (miRNA, siRNA, piRNA) sequencing uses next-generation sequencing technology to sequence (18-35 nt) nucleotide of particular tissue in certain physiological status. It not only identify and analyze known small RNA sequence, but also predict novel small RNA sequence and target gene associated.
The long chain non-coding RNAs (lncRNAs) refer to RNAs with length greater than 200nt and not involved in protein coding. LncRNA sequencing prepares samples with rRNA depletion protocol using Ribo-ZeroTM Series Kit, then generate sequence data to identify all lncRNA and mRNA for certain tissue or species in specific biological processes.
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