Third Generation Sequencing
PACBIO RS II
The PacBio RS II is a Single Molecule, Real-Time (SMRT®) DNA Sequencing System that provides the highest consensus accuracy and longest read lengths of any available sequencing technology.
SMRT technology based on the natural process of DNA replication: an active DNA polymerase, which immobilized at the bottom of each ZMW (zero-mode waveguides), attached itself to a strand of DNA to be replicated and examine the base at the point it is attached, which then identify the nucleotide and the whole sequence. In order to detect the base, each of the four nucleotides A, C, G and T are labeled with a different fluorescent dye having a distinct emission spectrum, enable real time observation of DNA synthesis.
SMRT Sequencing can use for de novo assembly, characterization of genetic variation, methylation analysis, microbiology studies, etc. It can also use for RNA Sequencing in order to study isoforms because of the extraordinarily long reads.
For more information, please find http://www.pacificbiosciences.com
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