Whole Genome Bisulfite Sequencing (WGBS)
WGBS can generate a genome-wide (to single base resolution) picture of DNA methylation with high accuracy. Giving that it is good to uncovering molecular mechanism, this can be used for embryonic development, evaluation of drug safety and researches focus on specific tissues or dynamic developmental processes.
Reduced Representation Bisulfite Sequencing (RRBS) - Only for Human and Mouse
RRBS is a bisulfite-based method, through enzyme digestion that enriches CG-rich regions of the genome (promoters and CpG islands) and bisulfite treatment, this reducing the sequencing data amount and sequencing cost required compare to WGBS, while it also provides single-nucleotide resolution and quantitative DNA methylation measurements.
Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) is used for study DNA methylation by the use of 5mC antibody. Through sequencing the enriched high methylation DNA area (especially the high CpG islands), it identifies the methylation status during analysis, by compare to the reference genome.
Chromatin Immunoprecipitation Sequencing (ChIP-Seq) is widely used to analyze protein-DNA interactions. Sequencing can detect precisely map global binding sites for any protein of interest, including transcription factors (TF) and histones, applied to study specific modifications and regulations.
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